Laboratory diagnosis

Diagnosed by

virus-specific antibodies

direct identification of viral material

HIV enzyme immunoassay-screening test--ELISA technique (99.9% sensitive) detection of IgG to envelope comp-gp20

Western-Blot---detects Antibody to HIV proteins--confirmatory test (99.9% specific)

HIV isolation--culture, p24 antigen detection by EIA or HIV genome detect by PCR

p24 antigen represents active HIV replication